Immortalized human podocyte cell line
WitrynaImmortalized Human Kidney Podocytes are developed by immortalizing primary human healthy kidney podocytes with SV40 Large T antigen. These kidney podocytes were … WitrynaCell line name: CIHP-1: Synonyms: Conditionally Immortalized Human Podocytes-1; AB8/13; AB 8/13; hiPOD; ciPOD: Accession: CVCL_W186: Resource Identification …
Immortalized human podocyte cell line
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WitrynaThe podocyte is a key cell in the glomerulus in health and disease: until recently, human podocytes have been difficult to study in vitro. We have developed a conditionally immortalized human podocyte cell line transfected with a temperature-sensitive simian virus 40 transgene: when the transgene is inactivated in vitro, these …
WitrynaATCC cell lines immortalized human podocytes ab8 13 m saleem Cell Lines Immortalized Human Podocytes Ab8 13 M Saleem, supplied by ATCC, used in … WitrynaCell Lines. HK2 cells (ATCC) were cultured in Dulbecco’s Modified Eagle Medium Low Glucose (DMEM LG) supplemented with 10% foetal bovine serum (FBS) (Euroclone S.p.A., Pero, MI, Italy). Immortalized human podocytes, previously characterized were cultured in DMEM High Glucose (Euroclone) with 10% FBS. Glomerular endothelial …
Witryna18 sie 2024 · A conditionally immortalized murine podocyte cell line (E11) was obtained from Cell Line Services. Podocytes were propagated at 33 °C in RPMI1640 supplemented with 10% FBS and 10 U/mL of IFN-γ (Saint Louis, MO, USA) to enhance expression of thermosensitive T antigen. ... Shimada, H.; Rajagopalan, L.E. Rho … Witryna1 mar 2010 · Evidence suggests that loss of podocytes into urine contributes to development of glomerular diseases; shed podocytes are frequently viable and proliferate in culture conditions. To determine the phenotypic characteristics of viable urinary cells derived from human subjects, we established long-term urinary cell …
Witryna25 mar 2024 · conditionally immortalized cell line. 1 reference. described by source. A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. 1 reference. A novel source of cultured podocytes. 1 reference. derived from organism type. Homo sapiens.
WitrynaMouse Podocyte Cell Line Mpc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... smart built agendaWitryna1 mar 2010 · The generation of podocytes cell lines from urine of patients and healthy volunteers is novel and will help to advance studies of podocyte cell biology, and further improvements in the approaches to cell transformation and/or cell culture techniques are needed to allow cultured podocytes to fully reproduce in vivo characteristics. … hill top medical centre birminghamWitryna[Creative-bioarray] Immortalized Cell Lines 소개 > BRIC ... ... hill top individual cottage ootyWitrynaG.J. Audesirk, in Comprehensive Toxicology, 2010 13.24.2.3.5 Immortal cell lines. Immortal cell lines are an alternative to primary cultures. Frequently used cell lines … smart built industrial ltdWitryna1 kwi 2002 · The conditionally immortalized human podocyte cell line AB8/13 was kindly provided by M. Saleem and has been described previously [8, 40]. Podocytes were cultured in the medium which contained ... smart bulb 360 customer reviewsWitrynaA breakthrough in cell biology research. Human telomerase reverse transcriptase (hTERT)–immortalized primary cells represent a breakthrough in cell biology … smart built pty ltdWitrynaTo investigate changes on the cellular level, we used an immortalized human podocyte cell line and generated a stable knockdown of Sphk2 (Sphk2-kd) by a lentiviral transduction method. smart builts shop